Intravital Microscopy

Back to list

Lung

Intravital in vivo imaging of the lung provides a powerful platform for studying dynamic pulmonary processes and respiratory function. By utilizing IVIM Technology's advanced imaging systems, researchers can gain critical insights into the cellular and molecular mechanisms underlying lung physiology and pathology, facilitating the development of innovative diagnostic and therapeutic strategies for respiratory diseases.

Real-time In Vivo Imaging of Cellular-level Dynamic Phenotype Inside the Living Body

  • IVIM Technology’s contract R&D service provides high-quality observations of cellular-level dynamics in various animal models.
  • Dynamic 3D imaging of various cellular-level dynamics such as cell trafficking, cell-cell interaction, and cell-microenvironment interaction inside the living body in vivo will provide you a new insight in the processes of human disease development.

Lung

RBC

Vessel

Intravascular Movement of Target Cells & Materials

  • In vivo real-time imaging of microcirculation
  • Microcirculation & dead space measurement
  • Functional capillary ratio measurement

Lung

Neutrophil

Vessel

Real-time imaging of various cell dynamics in vessels/lymphatics

  • Immune cells : Leukocyte, lymphocyte, granulocyte, neutrophil, macrophage, monocyte, dendritic cells, T cell, B cell, NK cell, microglial cell, etc.
  • Tissue cells : Vascular endothelial cells, lymphatic endothelial cell, hepatocyte, kidney cell, astrocyte, pericyte, etc.
  • Fluorescently labeled tumor cells (Mouse & human cell lines) : Brain/breast/lung/pancreatic cancer, glioblastoma, melanoma, etc.

Repetitive Monitoring of Dynamic Phenotype Changes

In Vivo visualization of dynamic molecular & cellular mechanisms

  • Cell differentiation, cell subpopulation
  • Dynamic remodeling of imaging targets

Lung

0.0 min

Neutrophil

Vessel

lung

3.0 min

lung

7.5 min

lung
lung
lung

Longitudinal In Vivo Imaging of Cellular-level Dynamic Phenotype Changes Inside the Living Body

  • IVIM Technology’s contract R&D service provides high-quality repetitive observations using imaging chambers.
  • Repetitive intravital imaging of cellular-level dynamics & tissue remodeling inside the living body in vivo will provide you a new insight in the processes of human disease development.

Dynamic Changes in Acute Injury Models
Immune cell dynamics in LPS-induced sepsis

  • Immune cell dynamics in multiple organ dysfunction syndrome
  • Immune cell sequestration in acute respiratory distress syndrome

In Vivo Monitoring of Drug Efficacy & Action

  • IVIM Technology’s contract R&D service provides a direct imaging analysis of delivery to target tissue and cells, efficacy, and mode of action (MOA) of new therapeutic candidates in microscopic cellular-level in various preclinical model of human disease.

Anti-inflammatory Therapeutic Candidates
Efficacy monitoring & MOA validation of anti-inflammatory therapeutic candidates

  • Disease model : Acute/chronic inflammation model, tumor model, etc.
  • Observations : Changes in microcirculation, tissue cell apoptosis, immune cell recruitment/motility/activity (phagocytosis), etc.
  • Tissue cells : Vascular endothelial cells, lymphatic endothelial cell, hepatocyte, kidney cell, astrocyte, etc.
  • Immune cells : Granulocyte, macrophage, neutrophil, dendritic cell, monocyte, microglial cell, NK cell, etc.

Real-time imaging of dynamic phenotype changes by treatment of therapeutic candidates

Pre-Drug

Neutrophil

RBC

Vessel

lung

Post-Drug

lung

Pre-Drug

RBC

lung

Post-Drug

lung

Lung

Sham

Merge

lung

Neutrophil

lung

RBC

lung

Vessel

lung

Lung

Vehicle

Merge

lung

Neutrophil

lung

RBC

lung

Vessel

lung

Lung

Drug

Merge

lung

Neutrophil

lung

RBC

lung

Vessel

lung
lung
lung
lung

IVM Imaging for Lung Research

  1. Pulmonary Immune Responses
  2. Respiratory Pathogen Dynamics
  3. Pulmonary Vascular Dynamics
  4. Lung Tumor Biology and Metastasis
  5. Drug Delivery and Pharmacokinetics

Lung

Intravital in vivo applications in the lungs of mice provide a powerful tool for studying pulmonary biology, disease processes, and therapeutic interventions, advancing our understanding of respiratory diseases and guiding the development of novel treatment strategies.

Here are some key applications that IVIM Technology can support you with:

1. Pulmonary Immune Responses

Intravital imaging allows for the visualization of immune cell dynamics within the lung tissue, including recruitment, activation, and interactions with pathogens or inflammatory stimuli. This aids in understanding immune responses to respiratory infections, inflammation, and autoimmune diseases affecting the lungs.

2. Respiratory Pathogen Dynamics

Researchers can use intravital imaging to study the behavior of respiratory pathogens, such as bacteria, viruses, and fungi, within the lungs of live mice. This facilitates the investigation of host-pathogen interactions, disease progression, and the development of novel antiviral or antibacterial therapies.

3. Pulmonary Vascular Dynamics

Intravital imaging enables the visualization of blood flow, vascular permeability, and endothelial cell behavior in the pulmonary vasculature. This provides insights into pulmonary vascular physiology, microvascular function, and the pathogenesis of pulmonary vascular diseases, including pulmonary hypertension and acute respiratory distress syndrome (ARDS).

4. Lung Tumor Biology and Metastasis

Intravital imaging allows researchers to study lung tumor growth, metastasis, and interactions with the lung microenvironment in real-time. This aids in understanding tumor progression, angiogenesis, and the efficacy of anti-cancer therapies targeting lung tumors.

5. Drug Delivery and Pharmacokinetics

Intravital imaging facilitates the visualization of drug distribution, uptake, and efficacy within the lung tissue. This enables researchers to optimize drug delivery strategies for respiratory diseases, such as asthma, chronic obstructive pulmonary disease (COPD), and lung cancer, and assess the pharmacokinetics of therapeutic agents in vivo.