Intravital Microscopy

The New All-in-One IVM Series

Confocal and Two-Photon Microscopy System for Live Animal Imaging

Intravital microscopy is a powerful imaging technique that allows real-time visualization of biological processes within living organisms.
Unlike traditional microscopy, which often involves studying fixed or isolated samples, intravital microscopy enables researchers observe dynamic events in their natural physiological context.
This technique is commonly employed in preclinical research in various fields including immunology, neuroscience, and cancer research, thus providing crucial insights into cellular behavior, interactions, and responses within living systems.

Intravital Microscopy Series 3

Have the opportunity to experience the world’s first All-in-One Intravital Microscopy Platform, Series 3, from IVIM Technology

The "New All-in-One IVM Series" stands as a game-changer addressing the critical need for effective cellular validation, tracking, and mode of action determination in real-time. It not only elevates academic research capabilities by providing cutting-edge tools for cellular exploration but also revolutionizes industrial research by expediting drug development timelines and improving overall success rates in the competitive pharmaceutical landscape.

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Features

Motion Compensation Function

Provides enhanced image quality of dynamic organs by automatically compensating for the effects of respiratory motions and brain pulses
Fast to Ultrafast Scanning

Allows users to track the movement of several cells in vivo to better understand the biological processes that are being examined
Customized Systems

Various apparatuses can be seamlessly integrated into the system depending on the research requirements and the researcher’s preferences
AI Noise-Canceling

IVIM's AI noise-canceling feature distinguish and eliminate noise, ensuring clearer and more accurate fluorescence signal detection
Animal Safety Check-up

allows continuous animal observation during imaging, facilitated by a camera inserted inside the system enclosure.
Temperature

allows check the animal's condition in real time by Feedback Heater Control, Body Plate Heater, Thermometer Sensor & Cover Glass Heater.

Products

IVM-C3

Tractable, Fast, Gentle

IVM-C3 is a highly integrated system for in vivo imaging, offering significantly enhanced detection efficiency, optical resolution, and image contrast compared to conventional fluorescence microscopy.
IVM-M3

Deep Tissue Imaging, High-Resolution, Tunable

IVM-M3 seamlessly combines the flexibility of a traditional converted microscope with the high-resolution imaging capabilities of second-harmonic generation microscopy.
IVM-CM3

High Contrast, High Resolution, Dual-mode, Tunable

IVM-CM3 delivers exceptional contrast and resolution with its tunable Two-Photon laser unit, allowing focus on desired wavelengths ranging from as low as 690 nm to higher up to 1050 nm, and everything in between.
IVM-MS3

Compact, Cost-Saving, Hands-Free

IVM-MS3 represents the smart evolution of IVM-M3, an All-in-One Two-Photon Intravital Microscopy system meticulously optimized for in vivo imaging.
IVM-CMS3

Cost-Effective, Straightforward, Dual-Mode

IVM-CMS3 stands out as the world's most compact and affordable dual-mode intravital confocal and two-photon microscope, offering versatile functionality in a single, streamlined box.
IVM-FS

Practical, Customizable

The IVM-FS system offers the same functions as the IVM-CMS3 system, with the key difference being its larger stage and modules, designed to accommodate animals larger than mice and rats (older than 6 weeks), such as ferrets, rabbits, and others. With the dark curtain provided, there's no need for a dedicated dark room or specific space just for the microscope.
AI-Image Denoiser

A Self-Supervised Learning Algorithm for Reliable Noise Reduction in Intravital Microscopy.
IVI Tag™ – In Vivo Labels

Track cells and proteins inside living organisms with unmatched clarity. Multi-color imaging, no dilution, no signal loss.
In Vivo Imaging Fixation Adjuncts

Stable, long-term imaging of dynamic organs with precision. From brain to pancreas, expand research possibilities without compromising animal welfare.

IVM-C3

Tractable, Fast, Gentle

IVM-C3 is a highly integrated system for in vivo imaging, offering significantly enhanced detection efficiency, optical resolution, and image contrast compared to conventional fluorescence microscopy.
IVM-M3

Deep Tissue Imaging, High-Resolution, Tunable

IVM-M3 seamlessly combines the flexibility of a traditional converted microscope with the high-resolution imaging capabilities of second-harmonic generation microscopy.
IVM-CM3

High Contrast, High Resolution, Dual-mode, Tunable

IVM-CM3 delivers exceptional contrast and resolution with its tunable Two-Photon laser unit, allowing focus on desired wavelengths ranging from as low as 690 nm to higher up to 1050 nm, and everything in between.
IVM-MS3

Compact, Cost-Saving, Hands-Free

IVM-MS3 represents the smart evolution of IVM-M3, an All-in-One Two-Photon Intravital Microscopy system meticulously optimized for in vivo imaging.
IVM-CMS3

Cost-Effective, Straightforward, Dual-Mode

IVM-CMS3 stands out as the world's most compact and affordable dual-mode intravital confocal and two-photon microscope, offering versatile functionality in a single, streamlined box.
IVM-FS

Practical, Customizable

The IVM-FS system offers the same functions as the IVM-CMS3 system, with the key difference being its larger stage and modules, designed to accommodate animals larger than mice and rats (older than 6 weeks), such as ferrets, rabbits, and others. With the dark curtain provided, there's no need for a dedicated dark room or specific space just for the microscope.
AI-Image Denoiser

A Self-Supervised Learning Algorithm for Reliable Noise Reduction in Intravital Microscopy.
IVI Tag™ – In Vivo Labels

Track cells and proteins inside living organisms with unmatched clarity. Multi-color imaging, no dilution, no signal loss.
In Vivo Imaging Fixation Adjuncts

Stable, long-term imaging of dynamic organs with precision. From brain to pancreas, expand research possibilities without compromising animal welfare.

Benefits

4-Color Simultaneous Imaging

The Intravital Microscope features four channels and detectors, enabling simultaneous visualization in four different colors, which greatly enhances research capabilities. This multiplexity allows for simultaneous monitoring of up to four labeled elements within the tissue.
Deep Tissue Imaging

In a two-photon microscope, the mechanism involves the fluorophore's simultaneous absorption of the photons within a few femtoseconds, leading to fluorophore excitation and subsequent light emission. Two-photon microscopy is particularly valuable for live cell imaging, especially in deeper and thicker tissues, surpassing the capabilities of confocal microscopy. Additionally, its second harmonic generation occurrence enhances axial and lateral resolution, rivaling confocal microscopy as pinholes are unnecessary. This makes two-photon microscopy a powerful tool for capturing high-quality images in diverse biological contexts.
In vivo Imaging apparatus for longitudinal Imaging

IVIM Technology offers intravital in vivo imaging apparatus for long-term imaging of various organs such as heart, lung, brain, abdominal organs, mammary tissue, pancreas and uterus in living mice. Our systems allow for continuous imaging sessions for several weeks post-exposure, minimizing the need for sacrificing animals after each session and promoting ethical research practices.
2D/3D/4D Imaging Data Visualization & Analysis

By using our software, users can observe movements over time in video format and easily visualize cross-sectional images from the side. Additionally, 3D reconstruction is also effortlessly achievable.

IVM-C3 (Confocal v.3)

IVM-C3 is a highly integrated system for in vivo imaging, offering significantly enhanced detection efficiency, optical resolution, and image contrast compared to conventional fluorescence microscopy. With a 4-wavelength laser and four high-sensitivity confocal detectors, IVM-C3 enables multi-dimensional views of living cells and tissues in 3D or 4D, while supporting up to four different colors.

Key Features

Tractable Fast Gentle

Bone Marrow

Transplanted cell Vessel (CD31)

Bone Marrow

Transplanted cell Vessel (CD31)

Bone Marrow

Transplanted cell Vessel (CD31)

In Vivo Imaging Fixation Adjuncts

IVM-CM3 (ConfocalandTwo-Photon v. 3)

IVM-CM3 delivers exceptional contrast and resolution with its tunable Two-Photon laser unit.It allows focus on samples at desired wavelengths ranging from as low as 690 nm to as high as 1050 nm, and everything in between. This cutting-edge system seamlessly combines the strengths of both Confocal and Two-Photon microscopy, offering limitless possibilities for three- dimensional imaging of living cells whether near the skin or deep within tumors in small animals. Explore the potential of IVM-CM3 for advanced and versatile imaging in your research.

Key Features

High Contrast High Resolution Dual-mode Tunable

Liver

Fibrotic collagen Hepatocyte

IVM-M3

IVM-MS3 (Two- Photon Smart v.3)

IVM-MS3 represents thesmart evolution of IVM-M3, an All-in-One Two-Photon Intravital Microscopy system meticulously optimized for in vivo imaging. This system seamlessly integrates a compact, high-stability, and maintenance-free fs-pulse laser unit into a single box. With the ability to focus on deep tissues at a fixed wavelength of 920nm, IVM- MS3 serves as an exceptional resource for researchers with specific targets, offering optimal functionality within limited resources and budget constraints. Explore the possibilities with IVM-MS3 for efficient and budget-friendly in vivo imaging.

Key Features

Compact Cost-Saving Hands-Free

Skin

Transplanted cell Transplanted cell Blood vessel (CD31)

Large Intestine

Immune cell Mannose (anti-inflammation) Blood vessel (CD31)

Kidney

H2B (Nuclei) ROSA-mT/mG Blood vessel (CD31)

IVM-CM3

IVM-CMS3 (Confocal and Two-Photon Smart v. 3)

IVM-CMS3 stands out as the world's most compact and affordable dual-mode intravital confocal and two-photon microscope, offering versatile functionality in a single, streamlined box. Leveraging the confocal laser units from IVM-C3 and the compact two-photon laser unit from IVM-MS3, IVM-CMS3 features a one-switch (one-click) mode changing capability, providing convenient multi-purpose use for intravital functional imaging while optimizing space and minimizing costs. Explore the efficient and cost-saving capabilities of IVM-CMS3 for your diverse imaging needs.

Key Features

Cost-Effective Straightforward Dual-Mode

Skin

CAG-RFP Collagen (SHG)

Liver

Collagen (SHG) Lipid Droplet (SF44)

Muscle

Sarcomere (SHG) Nerve (Thy1) Vessel (CD31)

IVM-MS3

AI-Image Denoiser

A Self-Supervised Learning Algorithm for Reliable Noise Reduction in Intravital Microscopy

The Research Challenge

Intravital microscopy allows researchers to capture dynamic biological events inside living organisms, such as immune cell migration, vascular changes, or rapid drug responses. To observe these processes in real time, imaging must be performed at very high speed.
However, faster imaging inevitably reduces the number of photons collected per frame, resulting in weaker signals and higher noise. This low signal-to-noise ratio (SNR) obscures cell boundaries, blurs fluorescence signals, and introduces uncertainty into quantitative analysis. For researchers, this means unreliable data, reduced reproducibility, and time lost repeating experiments.

The AI-Driven Solution

AI-Image Denoiser addresses these challenges by combining advanced spatio-temporal learning with self-supervised AI algorithms. Unlike conventional AI models that require clean reference images, it learns directly from noisy raw data.
By analyzing spatial and temporal patterns across frames, the software automatically separates true fluorescence signals from random noise, preserving the integrity of biological information.
This means researchers no longer need to prepare special datasets Research Benefits

Transforming Research Efficiency

Conventional denoising can take more than seven hours for a single dataset, slowing down research workflows and limiting the number of experiments that can be conducted.
AI-Image Denoiser reduces this process to just 30 minutes, enabling same-day analysis even for complex datasets. Beyond speed, the software preserves fluorescence without distortion, ensuring that fast-moving red blood cells or immune cells can be analyzed accurately. The result is not only higher efficiency but also improved reproducibility—researchers can trust that their quantitative measurements reflect true biological phenomena.

Applications in Advanced Imaging

Tracking immune cell migration in inflamed tissues
Monitoring rapid drug responses in vivo
Measuring blood flow dynamics through red blood cell movement
Long-term live imaging with reduced photobleaching

Validation in Real Experiments

In a transgenic mouse ear skin model, intravital microscopy was used to capture vascular structures and circulating red blood cells at high speed. The raw data exhibited strong noise due to limited exposure. After processing with AI-Image Denoiser, the resulting images revealed clear fluorescence signals without distortion and significantly reduced noise. This validation demonstrates that the software provides robust, trustworthy results even under demanding high-speed imaging conditions.

Specifications

Component	Minimum	Recommended
GPU	NVIDIA GeForce GTX 1080	NVIDIA GeForce RTX 4080
RAM	64 GB	128 GB
CPU	Intel Core i5-11600K, AMD Ryzen 5 3600	Intel Core i7-13700K, AMD Ryzen 9 5800X
Disk	512 GB SSD (≥150 GB free)	1 TB NVMe SSD (≥300 GB free)

IVM-FS

IVI Tag™ – In Vivo Labels

Track cells and proteins inside living organisms with unmatched clarity.
Multi-color imaging, no dilution, no signal loss.

Research Background

Traditional antigen-antibody labeling is limited in vivo due to dilution effects, handling errors, and signal loss. IVIM’s IVI Tag™ overcomes these barriers with advanced fluorescence tagging optimized for intravital microscopy. Researchers can now monitor vascular, immune, and neurological processes in real time without compromising signal integrity.

Core Technologies

Direct In Vivo Labeling – No dilution, no handling error, no fluorescence loss
Multi-Color Compatibility – Four excitation/emission channels + autofluorescence capture (up to 5 signals)
Organ & Cell-Specific Targets – Endothelial cells (CD31), lymphatic vessels (LYVE-1), dendritic cells (CD11b), T cells (CD3), macrophages (F4/80, CD206), neurons and astrocytes (GFAP, Tau, Amyloid β)
Broad Microscope Compatibility – Works with IVIM IVM series, confocal, and two-photon systems
DIY Labeling Kits – Conjugate antibodies, exosomes, peptides, or drugs for customized in vivo imaging

Research Benefits

Reliable in vivo tracking of immune dynamics, vascular remodeling, and disease progression
Longitudinal studies without repeated errors from traditional labeling
High reproducibility and quantitative accuracy for preclinical and neuroscience models
Easy integration into existing intravital microscopy workflows

Specifications

Excitation/Emission Wavelengths: 406/445, 495/519, 554/565, 651/667 nm
Compatibility: IVIM IVM systems, confocal, two-photon microscopes
Product Range: Antibody panels for CD31, LYVE-1, CD11b, Ly6C, Gr-1, Ly6G, CD3, CD45, F4/80, CD206, GFAP, Tau, Amyloid β, Iba1, and more

DIY In Vivo Labeling Kits

The IVI Tag™ DIY In Vivo Labeling Kit enables researchers to fluorescently tag their own targets—including antibodies, exosomes, peptides, and chemical drugs—for precise in vivo imaging. Using IVIM’s conjugation technology, the kit simplifies the labeling process into a few straightforward steps, making it possible to generate high-quality in vivo labels within hours. Unlike traditional antigen-antibody conjugation methods, IVI Tag™ DIY ensures that fluorophores are expressed at the intended target sites in living tissues, reducing variability and signal loss. This flexibility allows scientists to customize their experiments and track novel biological processes in real time.

In Vivo Label Preparation Steps

Conjugation