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Application: Inflammatory Bowel Disease Model

In vivo imaging of immune reaction response in inflammatory bowel disease

Abstract

In this experiment, we aimed to observe the immune reactivity of natural substances in an inflammatory disease animal model by monitoring immune responses over time in the colon lumen and around blood vessels. To observe the immune response, we made an incision in the peritoneum to expose the colon, then exposed the lumen to examine the activation of immune responses in the colon lumen and around blood vessels.

Therapeutic Target(s)

In an inflammatory bowel disease animal model, the purpose of treatment and drug administration is deeply related to understanding the pathological mechanisms of the disease, which include villus atrophy due to a massive increase in immune response. The primary focus is to monitor immune responses, emphasizing changes in immune biomarkers within the colon lumen and blood vessels.

Mouse Model

The use of DSS (dextran sulfate sodium) in an inflammatory bowel disease animal model induces villus atrophy in the colon through oral administration. A regimen of 5 cycles of DSS administration is necessary. This model is optimized for observing the initial increase in immune response within the colon, making it ideal for studying immune reactions via oral administration of DSS.

Experiment Design

Group #1 - Natural Product

Dosing: Receive Natural Product (5mg/kg) via oral administration.
Frequency: Oral administration for once
Sample Size: N=3

Group #2 - DSS + Natural Product

Dosing: Receive Natural Product (5mg/kg) with 2% DSS via oral administration.
Frequency: Oral administration for once
Sample Size: N=3

Duration

Total Time (Initiation to Completion): 1month

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Liver

In vivo intravital imaging of the liver offers a powerful approach to studying dynamic cellular and molecular processes within this vital organ. By leveraging intravital imaging technologies, researchers can gain valuable insights into the complex mechanisms underlying liver physiology and pathology, paving the way for the development of novel diagnostic and therapeutic approaches for liver diseases.

Here are some key applications:

1. Hepatic Microcirculation

Intravital imaging enables real-time visualization of blood flow dynamics, vascular permeability, and microvascular architecture within the hepatic microcirculation. Researchers can study the regulation of hepatic blood flow, sinusoidal perfusion, and vascular responses to physiological and pathological stimuli.

2. Hepatocyte Function

Imaging techniques allow for the direct observation of hepatocyte structure and function, including hepatocellular transport, bile secretion, and metabolic activities. This provides insights into the mechanisms of liver diseases such as fatty liver disease, hepatitis, and cirrhosis.

3. Kupffer Cell Dynamics

Intravital imaging facilitates the study of Kupffer cell behavior, phagocytic activity, and interactions with circulating immune cells within the liver sinusoids. Researchers can investigate the role of Kupffer cells in liver inflammation, immune responses, and clearance of pathogens or toxins.

4. Hepatic Fibrosis and Inflammation

Our imaging techniques allow for the visualization of hepatic fibrosis, inflammatory cell infiltration, and tissue remodeling within the liver parenchyma. This provides insights into the pathogenesis of liver diseases such as fibrosis, hepatitis, and autoimmune liver disorders.

5. Drug Metabolism and Hepatotoxicity

Intravital imaging can be used to evaluate drug metabolism, distribution, and hepatotoxic effects within the liver in real-time. Researchers can assess drug-induced liver injury, metabolic activation of prodrugs, and drug-drug interactions, guiding the development of safer and more effective therapeutics.

6. Liver Regeneration

Our imaging techniques enable the tracking of hepatocyte proliferation, liver regeneration, and tissue repair processes following injury or partial hepatectomy. Researchers can study the cellular and molecular mechanisms underlying liver regeneration and develop strategies to enhance hepatic recovery and function.

7. Tumor Microenvironment (TME)

Intravital imaging of liver tumors allows for the visualization of tumor-stroma interactions, angiogenesis, and immune cell infiltration within the tumor microenvironment. Researchers can study the dynamics of tumor growth, metastasis, and response to anti-cancer therapies, informing the development of novel treatment strategies for liver cancer.