Intravital Microscopy

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Heart

In vivo intravital imaging of the heart in mice offers a powerful approach to studying dynamic cardiac processes and cardiovascular function. By leveraging IVIM Technology's advanced imaging technologies, researchers can gain valuable insights into the dynamic processes underlying cardiac physiology and pathology, paving the way for the development of novel diagnostic tools and therapeutic strategies for heart diseases.

Real-time In Vivo Imaging of Cellular-level Dynamic Phenotype Inside the Living Body

  • IVIM Technology’s contract R&D service provides high-quality observations of cellular-level dynamics in various animal models.
  • Dynamic 3D imaging of various cellular-level dynamics such as cell trafficking, cell-cell interaction, and cell-microenvironment interaction inside the living body in vivo will provide you a new insight in the processes of human disease development.
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Real-time imaging of various cell dynamics in vessels/lymphatics

  • Immune cells : Leukocyte, lymphocyte, granulocyte, neutrophil, macrophage, monocyte, dendritic cells, T cell, B cell, NK cell, microglial cell, etc.
  • Tissue cells : Vascular endothelial cells, lymphatic endothelial cell, hepatocyte, kidney cell, astrocyte, pericyte, etc.
  • Fluorescently labeled tumor cells (Mouse & human cell lines) : Brain/breast/lung/pancreatic cancer, glioblastoma, melanoma, etc.

Longitudinal imaging of CX3 CR1+ cells and blood vessels in the same region of the heart of a Cx3 cr1gfp/+ mouse

IVM Imaging for Heart Research

  1. Cardiac Microcirculation
  2. Myocardial Structure and Function
  3. Cardiac Electrophysiology
  4. Vascular Remodeling and Angiogenesis
  5. Immune Cell Dynamics and Inflammation
  6. Cardioprotective Interventions
  7. Drug Delivery and Therapeutic Monitoring

Heart

In vivo intravital imaging of the heart in mice offers a powerful approach to studying dynamic cardiac processes and cardiovascular function. By leveraging IVIM Technology's advanced imaging technologies, researchers can gain valuable insights into the dynamic processes underlying cardiac physiology and pathology, paving the way for the development of novel diagnostic tools and therapeutic strategies for heart diseases.

Here are some key applications where IVIM Technology can provide support:

1. Cardiac Microcirculation

IVIM Technology enables real-time visualization of blood flow dynamics, capillary perfusion, and microvascular function within the cardiac microcirculation. Researchers can study the regulation of interventricular blood flow, endothelial function, and microvascular responses to ischemia, inflammation, and therapeutic interventions.

2. Myocardial Structure and Function

IVIM's advanced imaging techniques allow for the direct observation of myocardial structure and function, including cardiomyocyte contractility, sarcomere dynamics, and calcium handling. This provides insights into the mechanisms of cardiac contraction, relaxation, and electromechanical coupling under physiological and pathological conditions.

3. Cardiac Electrophysiology

IVIM Technology can support the study of cardiac electrophysiology, including action potential propagation, conduction velocity, and arrhythmogenic mechanisms within the heart. Researchers can investigate gap junctions, and cardiac excitability in normal cardiac rhythm and arrhythmia pathogenesis.

4. Vascular Remodeling and Angiogenesis

IVIM's imaging capabilities enable the visualization of vascular remodeling processes, angiogenic responses, and neovascularization within the ischemic or hypertrophic heart. Researchers can study the formation of collateral vessels, arteriogenesis, and angiogenic signaling pathways involved in cardiac adaptation and repair.

5. Immune Cell Dynamics and Inflammation

IVIM Technology allows for the tracking of immune cell trafficking, activation, and interactions within the cardiac tissue during inflammation, infection, and autoimmune diseases. Researchers can study the role of immune cells such as macrophages, neutrophils, and lymphocytes in cardiac inflammation, fibrosis, and remodeling.

6. Cardioprotective Interventions

IVIM's imaging techniques enable researchers to assess the efficacy of cardioprotective interventions, including pharmacological agents, stem cell therapies, and gene-based approaches, in real-time. IVIM Technology provides insights into the mechanisms of action, therapeutic targets, and potential adverse effects of cardioprotective strategies in the living heart.

7. Drug Delivery and Therapeutic Monitoring

IVIM Technology can be used to evaluate drug delivery, distribution, and therapeutic responses within heart tissue in vivo. Researchers can assess drug targeting strategies, pharmacokinetics, and cardiotoxic effects, guiding the development of novel therapies for cardiovascular diseases.