Newest Research on Intravital In Vivo Imaging IVIM TECHNOLOGY
NEWSLETTER |
ABOUT IVIM TECHNOLOGY
IVIM Technology, Inc. is a South Korea-based intravital confocal-two photon microscopy manufacturer and inhouse in vivo imaging service provider with its excellent expert research staff and customer service. |
Peptide labeling optimization for in vivo imaging instead of using synthetic peptides with chemical reactions
To visually check the internal mechanism and distribution of peptides in vivo through fluorescence bonding, chemical synthesis at the C-term or N-term part of the peptide sequence is performed. However, this method of chemical synthesis is inconvenient in that it is basically necessary to re-experiment the in vitro test results for the efficacy evaluation of the peptide and the internal biological reaction before fluorescent coupling. IVIM Technology has optimized the way to utilize the internal mechanism and distribution of fluorescence-coupled peptides with a biomicroscope without changing their original efficacy properties.
|
“IVIM Technology’ s unique peptide labeling method optimized for in vivo imaging thorough bio-conjugation rather than a chemical synthesis makes possible in real-time, long time monitoring of peptide distributiton mechanism and efficacy under a biological microscope.” |
The NHS (N-Hydroxy Succinimide) ester reaction technique is usually used for fluorescent labeling, fluorescent materials, and various fluorescent light-emitting materials to review in vivo bio-molecular mechanisms and chemical reactions. The reaction between these NHS ester groups and amino groups depends on pH where the optimal pH concentration is 8.3 to 8.5.
|
Most of the NHS ester group is dissolved in water. Still, the optimal reaction design is sometimes completed by dissolving it with dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and adjusting the pH to 8.3 to 8.5. In the case of NHS ester amine reaction, one of the recent technique is the NHS ester modification which is formed by a chemical reaction between an ester group and amine group that is also used for the linker part of the antibody drug conjugate. It has a technical advantage that is widely used in fluorescence labeling of peptides. |
Time-dependent Imaging of Peptide-Labeled Substances with Bioreactions |
For time-dependent imaging of NHS ester bioconjugation drugs to peptides, the Cranial Imaging Windows (CIW) animal model can be utilized. CIW animal model that enables real-time observation of neuroinflammatory responses in the cerebral cortex, brain tumors, and neurovascular changes by imaging through a window in a living animal. Through the IVM-CM3, which is capable of confocal and two-photon mode imaging that boasts real-time laser scanning speed, it was possible to image the cerebral cortex of a mouse with a cranial imaging window inserted to the extent that observation at the cellular level. With the Z-axis cross-sectional imaging function, images up to a depth of 200 μm in the brain could be acquired with a confocal mode microscope and very clearly visualized by time. |
The distribution of peptides can be graphed and quantified by in vivo imaging over time and then calculating absorbance for peptide fluorescence under a biomicroscope. Statistical analysis of graphs utilizes various statistical programs such as Sigma Plot, Graph Prism, and Origin to obtain scientific and objective results. |
In addition to peptides, low-molecular compounds such as nanoparticles and exosomes can also be imaged in vivo. |
At IVIM Technology, researchers specializing in vivo imaging of nanoparticles and exosomes, which are small-molecular compounds other than peptides, directly perform bioimaging to meet the needs of researchers. For more information, you can visit our website, www.ivimtech.com, or email us with any questions. |
|
