Newsletter for Intravital In Vivo Imaging Volume 1 Issue 1, Dec 2022 |
INTRAVITAL IN VIVO IMAGING
USING AN IMAGING WINDOW OR CHAMBER
For in vivo longitudinal monitoring, the imaging window chambers such as dorsal skinfold chamber for in vivo analysis of cancer or drug candidates, cranial imaging window for brain imaging, and abdominal window for imaging internal organs can be utilized.
“In vivo imaging chambers are best optimized by who is both the manufacturer of the microscope and have the widest application of it for real-time use.”
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Thanks to its engineers and the know-how gained in the field with its application specialists, IVIM Technology continuously optimizes its in vivo imaging techniques and apparatus such as chambers. In this way, the live animal is less affected by surgery for imaging, and the intravital imaging result is more realistic.
In this issue, we will take a look at three of the imaging chambers and techniques IVIM has optimized. |
Dorsal Skinfold Chamber is used for non-invasive analyses of quantitative assessments of dynamic process in the preclinical models. By optimizing the chamber, implantation, and imaging process, it is possible to observe the same spot in one single animal for up to several months |
For a case study by our research group, DSC was utilized for in vivo analysis of tumor development for longitudinal cancer xenograft imaging in one single mouse model over time. For a tumor model, tumor cells were implanted first and the tumor development on the site was observed. We could find that in the drug-treated group, the tumor vessels were less dilated compared to the normal group. After drug treatment, vessel morphology becomes more normalized compared to the sham mouse model. Finally, we validated the in vivo efficacy of anti-angiogenic drug by visualizing together with tumor vessels. |
For longitudinal in vivo imaging of mouse brain, we utilize a cranial imaging window to visualize cerebral cortex. In a live window, it is possible to monitor the real-time fast dynamic of brain neuroinflammatory responses, brain tumor or the behavior of neurovascular unit in the cerebral cortex. |
For example, in a case study with the IVM-CM imaging system, video-rate laser-scanning confocal & two-photon microscopy, we achieved the high-quality cellular-level visualization of the brain cortex of a cranial imaging window implanted mouse model. By z-stack optical section imaging, z-stack images of the brain with a depth of about 200 μm were obtained with confocal mode microscopy. |
In the maximal intensity z-projection and mosaic image, brain vasculature and blood vessel lumen were clearly visualized by simultaneous fluorescence detection of CD31+ endothelial cells (red) and FITC-dextran (green) flowing within the blood vessels, respectively. By converting the imaging mode from confocal mode to the two-photon mode in the IVM-CM imaging system, collagen fiber (cyan) signals from the second harmonic generation with vasculature were successfully visualized within the same field of view as in the confocal mode. |
Abdominal Imaging Chamber |
n vivo cell dynamics and development of abdominal organs such as pancreas, kidney and spleen can be observed utilizing an abdominal imaging window on a long-term basis. For example, by utilizing the abdominal imaging window, we could visualize real-time cellular dynamics of endogenous monocytes and dendritic cell in spleen and also pancreatic islet b cell using MIP-GFP Tg mouse. |
Helping you achieve of your research goals |
IVIM Technology, Inc. addresses the needs of individual research topics for intravital in vivo imaging. With its expert research staff specialized in in vivo surgery and imaging, IVIM Technology also provides the training courses for animal handling, in vivo fluorescence labeling kit use and optimization of the purpose-built chamber model. For more information, visit our website www.ivimtech.com or send us an email for any inquiries. |
IVIM TECHNOLOGY. All Rights Reserved.
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