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역동적으로 살아 움직이는 장기의 영상화가 가능케하는

아이빔테크놀로지의 조직 운동 안정기(TMS)

Minimizing Subtle Micron-level Movements

  The primary challenge in imaging dynamic organs is minimizing subtle micron-level movements that can obstruct clear observation. As a pioneering inovative solution, to overcome the difficulties associated with tissue motion, we have developed a tissue motion stabilizer (TMS) uses negative pressure to securely hold pumping organs in place, ensuring stable imaging conditions and minimizing motion artifacts. By applying suction to the tissue, the TMS system restrains motion, allowing for high-quality imaging even in dynamic environments. It features a circular metal fixture and a suction pump that work together to stabilize the observation site and mitigate the impact of pulsations (Fig. 1).

Figure 2: 조직 운동 안정기(TMS)를 이용한 살아있는 실제 동물모델의 의 영상화 모습

In Vivo Lung Imaging of Live Mouse Utilizing TMS

Figure 4: Neutrophil microcirculation in the lung of live mouse

Figure 5: Real-time imaging of immune cell dynamics in the heart of live mouse

  Unlike the relatively soft and deformable lung tissue, the heart, being deeper in the body, requires stronger negative pressure for effective visualization. In this example, LysM-eGFP transgenic mice were used to visualize immune cell dynamics, with vascular labeling achieved through intravenous injection of anti-CD31 antibody and DiD-labeled red blood cells (RBCs). After a chest incision exposed the cardiac tissue, an imaging window chamber with a vacuum-based tissue motion stabilizer set to 890–920 mbar was used to stabilize the tissue and enable high-quality heart imaging.

Ref: European Heart Journal - Imaging Methods and Practice (2024) 2, qyae062  

In Vivo Thymus Imaging of Live Mouse Utilizing TMS

Figure 6: Real-time imaging of immune cell behavior and thymic vasculature dynamics in live mice

조직 운동 안정기(TMS)를 이용한 살아있는 동물모델의 생체 내 자궁 영상화 

Figure 7: Animal mounting for uterus imaging in live mice

  The natural rhythmic contractions of the uterus have historically posed significant challenges for capturing high-quality intravital images, often resulting in blurry or unclear images with traditional methods. Our Uterus Imaging System addresses these issues with a specially designed vacuum-suction chamber (Fig.1) that incorporates several innovative modifications to stabilize the uterus during imaging. This advanced chamber effectively mitigates the effects of uterine contractions, enabling researchers to achieve clear, high-resolution, and consistent imaging at the same location. The uterus is composed of muscular tissue, enabling it to sustain pregnancy and facilitate childbirth. Throughout these processes, it is crucial for the uterus to receive an adequate blood supply. By observing the muscular tissue and blood vessels (Fig. 7) in the uterus of mice before and during pregnancy, we obtained results that enhance our understanding of the structural changes in the uterus due to pregnancy and the diseases that may occur during pregnancy.

Figure 8: Uterine wall of imaging results of live mice

Previous Events

Mar 28-30 Annual Meeting of the Physiological Society of Japan – Exhibition

Apr 5-10 AACR (American Association of Cancer Research) 2024 – Exhibition

May 7-11 ASGCT (American Society of Gene & Cell Therapy) 2024

Jun 1-3 China Life Science Conference 2024 and China Guangzhou International Life Science Expo

July 2-5 IVBM (International Vascular Biology Meeting 2024)