Repetitive Intravital Imaging
Repetitive Monitoring of Dynamic Phenotype Changes
In Vivo visualization of dynamic molecular & cellular mechanisms
- • Cell differentiation, cell subpopulation
- • Dynamic remodeling of imaging targets
Bone Marrow
Actin
Nuclei
Sinus
30 min
84 min
Small intestine
Fatty acid
Lacteal
3.5 sec
7.3 sec
Longitudinal In Vivo Imaging of Cellular-level Dynamic Phenotype Changes Inside the Living Body
- • IVIM Technology’s contract R&D service provides high-quality repetitive observations using imaging chambers.
- • Repetitive intravital imaging of cellular-level dynamics & tissue remodeling inside the living body in vivo will provide you a new insight in the processes of human disease development.
Dynamic Changes in Acute Injury Models
Immune cell dynamics in LPS-induced sepsis
- • Immune cell dynamics in multiple organ dysfunction syndrome
- • Immune cell sequestration in acute respiratory distress syndrome
Lung
0.0 min
Neutrophil
Vessel
3.0 min
7.5 min
Immune cell behaviors in various ischemia-reperfusion injury models (hepatic / renal / myocardial ischemia-reperfusion, ischemic stroke, hindlimb ischemia)
Liver
Reperfusion 0 h
Neutrophil/Kupffer cell
Hepatocyte
Reperfusion 6 h
Reperfusion 24 h
Immune cell recruitment to irritated spot
- • Cell dynamics in irritated tissue after high-power laser irradiation
- • Wound-healing process at puncture
- • Cell dynamics at toxic chemical-induced injury site
Skin
Nonirradiated
Neutrophil
Vessel
Hair fcllicle
Before irradiation
6 h after irradiation
Dynamic Changes in Chronic Disease Models
Phenotype changes in MCD (methionine and choline deficient)-diet induced NAFLD/NASH (nonalcoholic fatty liver/nonalcoholic steatohepatitis)
- • Hepatic lipid droplet accumulation
- • Immune cell recruitment, hepatocyte necrosis/apoptosis
Liver
Normal diet
Lipid droplet
Vessel
MCD Day 2
MCD Day 21
Phenotype changes in liver/lung fibrosis model
- • Excessive collagen deposition
- • Fibrotic stage (number of septa, etc.)
- • Immune cell recruitment, hepatocyte/alveolar epithelial cell death
Liver
Fibrotic collagen
Hepatocyte
Fibrotic collagen
Hepatocyte
Fibrotic collagen
Lipid droplet
Dynamic Changes in Tumor Models
Changes in vascular morphology and vascular permeability
Tumor cell growth, tumor cell death
Tumor cell dissociation
Formation of circulating tumor cells, tumor metastasis
Tumor
Day 7
Vessel
Lung cancer cell
Day 10
Day 13
Vessel morphology
Vessel
Lung cancer cell
Vascular permeability
Vessel
Dye
Lung cancer cell
Tumor cell dissociation
Breast cancer cell
Stromal cell
Dynamic Changes in Brain Disease Models
Changes in BBB penetration
Changes in target accumulation in brain tissue
Changes in cortical capillaries, vascular permeability & vessel diameter
Changes in pericyte coverage
Immune cell recruitment to inflammatory / damaged spot
Tissue cell apoptosis & phagocytosis
Brain
Day 7
Vessel / BBB leakage
Day 14
Day 21
Brain
Sham
Apoptotic body
Vessel
Neuroinflammation
Apoptotic body
Vessel
Brain
Day 4
Neutrophil/Macrophage
Vessel
Day 10