Repetitive Intravital Imaging
Repetitive Monitoring of Dynamic Phenotype Changes
In Vivo visualization of dynamic molecular & cellular mechanisms
- • Cell differentiation, cell subpopulation
- • Dynamic remodeling of imaging targets
Bone Marrow
Actin
Nuclei
Sinus
30 min

84 min

Small intestine
Fatty acid
Lacteal
3.5 sec

7.3 sec

Longitudinal In Vivo Imaging of Cellular-level Dynamic Phenotype Changes Inside the Living Body
- • IVIM Technology’s contract R&D service provides high-quality repetitive observations using imaging chambers.
- • Repetitive intravital imaging of cellular-level dynamics & tissue remodeling inside the living body in vivo will provide you a new insight in the processes of human disease development.
Dynamic Changes in Acute Injury Models
Immune cell dynamics in LPS-induced sepsis
- • Immune cell dynamics in multiple organ dysfunction syndrome
- • Immune cell sequestration in acute respiratory distress syndrome
Lung
0.0 min
Neutrophil
Vessel

3.0 min

7.5 min



Immune cell behaviors in various ischemia-reperfusion injury models (hepatic / renal / myocardial ischemia-reperfusion, ischemic stroke, hindlimb ischemia)
Liver
Reperfusion 0 h
Neutrophil/Kupffer cell
Hepatocyte

Reperfusion 6 h

Reperfusion 24 h

Immune cell recruitment to irritated spot
- • Cell dynamics in irritated tissue after high-power laser irradiation
- • Wound-healing process at puncture
- • Cell dynamics at toxic chemical-induced injury site
Skin
Nonirradiated
Neutrophil
Vessel
Hair fcllicle

Before irradiation

6 h after irradiation

Dynamic Changes in Chronic Disease Models
Phenotype changes in MCD (methionine and choline deficient)-diet induced NAFLD/NASH (nonalcoholic fatty liver/nonalcoholic steatohepatitis)
- • Hepatic lipid droplet accumulation
- • Immune cell recruitment, hepatocyte necrosis/apoptosis
Liver
Normal diet
Lipid droplet
Vessel

MCD Day 2

MCD Day 21

Phenotype changes in liver/lung fibrosis model
- • Excessive collagen deposition
- • Fibrotic stage (number of septa, etc.)
- • Immune cell recruitment, hepatocyte/alveolar epithelial cell death
Liver
Fibrotic collagen
Hepatocyte

Fibrotic collagen
Hepatocyte

Fibrotic collagen
Lipid droplet


Dynamic Changes in Tumor Models
Changes in vascular morphology and vascular permeability
Tumor cell growth, tumor cell death
Tumor cell dissociation
Formation of circulating tumor cells, tumor metastasis
Tumor
Day 7
Vessel
Lung cancer cell

Day 10

Day 13

Vessel morphology
Vessel
Lung cancer cell

Vascular permeability
Vessel
Dye
Lung cancer cell

Tumor cell dissociation
Breast cancer cell
Stromal cell

Dynamic Changes in Brain Disease Models
Changes in BBB penetration
Changes in target accumulation in brain tissue
Changes in cortical capillaries, vascular permeability & vessel diameter
Changes in pericyte coverage
Immune cell recruitment to inflammatory / damaged spot
Tissue cell apoptosis & phagocytosis
Brain
Day 7
Vessel / BBB leakage

Day 14

Day 21

Brain
Sham
Apoptotic body
Vessel

Neuroinflammation
Apoptotic body
Vessel


Brain
Day 4
Neutrophil/Macrophage
Vessel

Day 10

