IVM-C3 (Confocal v.3)
Tractable, Fast and Gentle
IVM-C3 is a highly integrated intravital microscopy for in vivo imaging with an enormously increased detection efficiency, optical resolution, and contrast of the image compared to conventional fluorescence microscopy. Equipped with a 4-wavelength laser and four high-sensitivity confocal detectors, IVM-C3 allows multi-dimensional views of living cells and tissues in 3D or 4D up to four different colors. It is the optimal system for simultaneously observing various dynamic multi-cellular behaviors in small live animal models.
- • Multi-color Simultaneous Imaging (4 channels, 4 different colors)
- • Fully Integrated in vivo Maintenance Unit / Animal Stage
(e.g., Monitoring & Homeostatic Regulation of Animal Vitality) - • Ultra High-Speed Imaging (max. 100 fps - 512x512 pixels)
- • 4D Animal Motion Compensation (XYZ & Time)
Intravital imaging of mCLING-labeled exosome uptake into neutrophils (LysMgfp/+; Ly6G+) in the liver of sham mice
Representative time-lapse imaging of mCLING-labeled exosomes inside the Ly6G+ neutrophil cells of the liver
IVM-C3 | IVM-M3 | IVM-CM3 | IVM-MS3 | IVM-CMS3 | |
---|---|---|---|---|---|
Laser | |||||
Type | Confocal Laser Unit | Tunable Two-Photon Laser Unit Ti:Sapphire laser |
Confocal Laser Unit Tunable Two-Photon Laser Unit Ti:Sapphire laser |
Compact Two-Photon Laser Unit | Confocal Laser Unit Compact Two-Photon Laser Unit |
Wavelength | 405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW) |
690 – 1,050 nm | For Confocal
405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW) For Two-Photon 690-1,050 nm |
Fixed 920 nm | For Confocal
405 nm (20mW), 488 nm (20mW), 561 nm (20mW), 640 nm (20mW) For Two-Photon Fixed 920 nm |
Fluorescence | |||||
Detector | Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole |
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) |
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) |
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) |
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) 25-2,000 μm variable pinhole Two-Photon Detector Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.) 4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current) |
- Variable Emission Filter
(Optional) - 6 or 2 emission filters can be mounted on each of four detectors
- Scanner
- Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
- Objectives
- Max. 5 objectives are mountable on S/W controlled motorized turret
(1X – 100X)
Compatible for commercial objectives
- FOV
- 100 x 100 ㎛ ² – 10 x 10 mm²
- Pixel Resolution
- Max. 2,048 x 2,048 pixels
- Imaging Speed
- Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels
- 3D Stage
- Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
- 4D In Vivo Imaging
Motion Compensation - XY motion compensation : Averaged image acquisition with motion
artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Controllable by IVM Engine software
- Live Animal
Maintenance Unit - Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
- In Vivo Imaging
Chamber SET - Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Heart Imaging Window SET
- Inhalation Anesthesia
System - Rodent Animal Inhalation Anesthesia System
Anesthesia Mask and Connections
- Image Display
- Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
- In Vivo Imaging Mode
- Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)
