The most compact Two-Photon System in the world

IVM-MS2 is the All-in-One IntraVital Two-Photon Microscopy System, optimized for  in vivo imaging experiments and equipped with a new compact high-efficiency fs-pulse laser module. Especially, because it integrates a compact high-stability  maintenance-free fs-pulse laser into a single box, the IVM-MS2 is the ideal solution  for customers in need of a two-photon microscope with limited resources of  space and budget.

  • World’s 1st all-in-one IntraVital Microscopy for live animal model
  • Ultra High-speed Imaging (max. 100 fps – 512×512 pixels)
  • 4D Animal Motion Compensation (X,Y,Z & Time)
  • Simple hand-free turn-key operation of 920 nm NIR fs-laser for deeper tissue imaging
  • Less expensive, fully-automated fs-laser system
  • Space saving with no additional fs-laser box configuration
  • Simple hand-free turn-key operation of the fs-laser for two-photon excitation
  • Easy maintenance and management without liquid cooling system requirements

Whole Tissue 3D Imaging with Tissue Clearing Technique

IVM-C IVM-M IVM-CM IVM-MS2
Laser
Type Confocal Laser Unit Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Confocal Laser Unit
Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Compact Two-Photon Laser Unit
Wavelength 405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
690 – 1,050 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
690-1,050 nm
Fixed 920 nm
Fluorescence
Detector Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Variable Emission
Filter (Optional)
6 or 2 emission filters can be mounted on each of four detectors
ScanHead
Scanner Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives
Image
FOV 100 x 100 ㎛ ² – 10 x 10 mm²
Pixel Resolution Max. 2,048 x 2,048 pixels

Imaging Speed Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels

In Vivo Animal Stage
3D Stage Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
4D In Vivo Imaging Motion Compensation XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Additional In Vivo Modules
Live Animal Maintenance Unit Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
In Vivo Imaging Chamber SET Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Inhalation Anesthesia System Rodent Animal Inhalation Anesthesia System
Engine & Studio Software
Image Display Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
In Vivo Imaging Mode Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)