IVM-MS2 (Two-Photon Smart Ver. 2)

The most compact Two-Photon System in the world

IVM-MS2 is the All-in-One IntraVital Two-Photon Microscopy System, optimized for in vivo imaging experiments and equipped with a new compact high-efficiency fs-pulse laser module. Especially, because it integrates a compact high-stability maintenance-free fs-pulse laser into a single box, the IVM-MS2 is the ideal solution for customers in need of a two-photon microscope with limited resources of space and budget.

  • • World’s 1st all-in-one IntraVital Microscopy for live animal model
  • • Ultra High-speed Imaging (max. 100 fps – 512×512 pixels)
  • • 4D Animal Motion Compensation (X,Y,Z & Time)
  • • Simple hand-free turn-key operation of 920 nm NIR fs-laser for deeper tissue imaging
  • • Less expensive, fully-automated fs-laser system
  • • Space saving with no additional fs-laser box configuration
  • • Easy maintenance and management without liquid cooling system requirements
  • Adipose Tissue
    Nuclei
    Actin
  • Skeletal Muscle
    Nuclei
    Actin
    SHG
  • Lung
    Nuclei
    Actin
    SHG
  • Ear Skin
    Actin
    SHG

Whole Tissue 3D Imaging with Tissue Clearing Technique

Specification
IVM-C IVM-M IVM-CM IVM-CMS IVM-MS2
Laser
Type Confocal Laser Unit Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Confocal Laser Unit
Tunable Two-Photon Laser Unit

Ti:Sapphire laser
Confocal Laser Unit
Compact Two-Photon Laser Unit
Compact Two-Photon Laser Unit
Wavelength 405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
690 – 1,050 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
690-1,050 nm
For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
Fixed 920 nm
Fixed 920 nm
Fluorescence
Detector Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5,
Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High
Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5,
Cy5.5, etc.)
4 High quantum efficiency PMTs
(UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Variable Emission Filter
(Optional)
6 or 2 emission filters can be mounted on each of four detectors
ScanHead
Scanner
Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives
Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives
Image
FOV
100 x 100 ㎛ ² – 10 x 10 mm²
Pixel Resolution
Max. 2,048 x 2,048 pixels
Imaging Speed
Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels
In Vivo Animal Stage
3D Stage
Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
4D In Vivo Imaging
Motion Compensation
XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Additional In Vivo Modules
Live Animal
Maintenance Unit
Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
In Vivo Imaging
Chamber SET
Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Inhalation Anesthesia
System
Rodent Animal Inhalation Anesthesia System
Engine & Studio Software
Image Display
Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
In Vivo Imaging Mode
Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)