Dual Mode Imaging Platform

IVM-CM is the All-in-One IntraVital Confocal / Two-Photon Microscopy System, optimized for in vivo imaging experiments. Especially, because both of the confocal and two photon microscopes are integrated into a single-box packaged system, IVM-CM provides the ultimate versatile functionality of the IVM-C and IVM-M in one system.

  • World’s 1st all-in-one IntraVital Microscopy for live animal model
  • Ultra High-speed Imaging (max. 100 fps – 512×512 pixels)
  • 4D Animal Motion Compensation (X,Y,Z & Time)
  • Laser wavelength tuning for optimal two-photon excitation of a wide-range of fluorescence agents
  • Deeper tissue imaging with a longer-wavelength NIR fs-laser system
  • One-click automated transition between Confocal and Two-Photon imaging mode
  • Dual-mode IVM-C (Confocal) / IVM-M (Two-Photon) imaging capability
IVM-C IVM-M IVM-CM IVM-MS2
Laser
Type Confocal Laser Unit Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Confocal Laser Unit
Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Compact Two-Photon Laser Unit
Wavelength 405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
690 – 1,050 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
690-1,050 nm
Fixed 920 nm
Fluorescence
Detector Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Variable Emission
Filter (Optional)
6 or 2 emission filters can be mounted on each of four detectors
ScanHead
Scanner Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives
Image
FOV 100 x 100 ㎛ ² – 10 x 10 mm²
Pixel Resolution Max. 2,048 x 2,048 pixels

Imaging Speed Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels

In Vivo Animal Stage
3D Stage Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
4D In Vivo Imaging Motion Compensation XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Additional In Vivo Modules
Live Animal Maintenance Unit Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
In Vivo Imaging Chamber SET Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Inhalation Anesthesia System Rodent Animal Inhalation Anesthesia System
Engine & Studio Software
Image Display Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
In Vivo Imaging Mode Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)