IVM-C (Confocal)

In Vivo Live Cell Imaging Platform

IVM-C is the All-in-One IntraVital Confocal Microscopy System, optimized for in vivo imaging experiments. Especially, because it is equipped with a 4-wavelength laser and 4 high-sensitivity confocal detectors, IVM-C is the optimal system to observe highly diverse, dynamic multi-cellular behaviors in live animals simultaneously with 4 different fluorescence colors.

  • • World’s 1st all-in-one IntraVital Microscopy for live animal model Fully Integrated In Vivo Maintenance Unit / In Vivo Animal Stage
    (e.g. Monitoring & Homeostatic Regulation of Animal Vitality)
  • • Ultra High-speed Imaging (max. 100 fps - 512x512 pixels)
  • • 4D Animal Motion Compensation (X,Y,Z & Time)
  • • Customizable design for modifications according to the customer’s requests and accommodations for future updates
  • • Easy & highly efficient multi-color simultaneous imaging
  • • High flexibility in selection of laser / detector wavelength
  • • Applicability for conventional ex vivo , in vitro and in vivo imaging

Intravital imaging of mCLING-labeled exosome uptake into neutrophils (LysMgfp/+; Ly6G+) in the liver of sham mice

Representative time-lapse imaging of mCLING-labeled exosomes inside the Ly6G+ neutrophil cells of the liver

Exosome-based delivery of super-repressor IkBa relieves sepsis-associated organ damage and mortality, Science Advances (2020)
Specification
IVM-C IVM-M IVM-CM IVM-CMS IVM-MS2
Laser
Type Confocal Laser Unit Tunable Two-Photon Laser Unit
Ti:Sapphire laser
Confocal Laser Unit
Tunable Two-Photon Laser Unit

Ti:Sapphire laser
Confocal Laser Unit
Compact Two-Photon Laser Unit
Compact Two-Photon Laser Unit
Wavelength 405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
690 – 1,050 nm For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
690-1,050 nm
For Confocal
405 nm (20mW), 488 nm (20mW),
561 nm (20mW), 640 nm (20mW)
For Two-Photon
Fixed 920 nm
Fixed 920 nm
Fluorescence
Detector Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Confocal Detector
Wavelength: 185 – 900 nm (DAPI, CFP, GFP, YFP, RFP, Cy5,
Cy5.5, etc.)
4 Ultra-broadband high SNR PMTs (UV to Near IR, Ultra High
Sensitivity, Low Dark Current)
25-2,000 μm variable pinhole (16 steps)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5,
Cy5.5, etc.)
4 High quantum efficiency PMTs
(UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Two-Photon Detector
Wavelength: 185 – 760 nm (DAPI, CFP, GFP, YFP, RFP, Cy5, Cy5.5, etc.)
4 High quantum efficiency PMTs (UV to Near IR, Ultra High Sensitivity, Low Dark Current)
Variable Emission Filter
(Optional)
6 or 2 emission filters can be mounted on each of four detectors
ScanHead
Scanner
Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives
Max. 5 objectives are mountable on S/W controlled motorized turret (1X – 100X)
Compatible for commercial objectives
Image
FOV
100 x 100 ㎛ ² – 10 x 10 mm²
Pixel Resolution
Max. 2,048 x 2,048 pixels
Imaging Speed
Standard : 30 fps @ 512 x 512 pixels
(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels
In Vivo Animal Stage
3D Stage
Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
4D In Vivo Imaging
Motion Compensation
XY motion compensation : Averaged image acquisition with motion artifact compensation
Z motion compensation : Image-based sample Z position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
T motion compensation : Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Additional In Vivo Modules
Live Animal
Maintenance Unit
Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater
In Vivo Imaging
Chamber SET
Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Inhalation Anesthesia
System
Rodent Animal Inhalation Anesthesia System
Engine & Studio Software
Image Display
Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)
In Vivo Imaging Mode
Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)